Journal: Oncogene

Article Title: Inhibition of EGFR pathway signaling and the metastatic potential of breast cancer cells by PA-MSHA mediated by type 1 fimbriae via a mannose-dependent manner.

doi: 10.1038/onc.2010.70

Figure Lengend Snippet: Figure 5 PA-MSHA inhibits the invasive abilities of MDA-MB-231HM and MDA-MB-468 Cells. (A) Western blot analyses of the expression of VEGF, MMP-9, Cathepsin D and E-Cadherin in PBS, PA-, or PA-MSHA-treated cells. (B) Quantification of VEGF (a) and MMP-9 (b) in conditioned cell supernatant using ELISA. *Po0.05 vs PA-treated control group. Bars, s.d.

Article Snippet: In addition, the amounts of mouse VEGF and MMP-9 in PA-MSHA-treated PA-treated MDA-MB-231HM and MDAMB-468 5-week xenografts were also determined by an ELISA kit (Mouse VEGF SXR068, Mouse MMP9 SXR049 immunoassay kit, R&D Systems Inc.) in duplicate according to the manufacturer’s instructions.

Techniques: Western Blot, Expressing, Enzyme-linked Immunosorbent Assay, Control

Journal: Oncogene

Article Title: Inhibition of EGFR pathway signaling and the metastatic potential of breast cancer cells by PA-MSHA mediated by type 1 fimbriae via a mannose-dependent manner.

doi: 10.1038/onc.2010.70

Figure Lengend Snippet: Figure 6 Effect of the administration of PA-MSHA on tumor growth and lung micrometastatic expansion of MDA-MB-231HM and MDA- MB-468 Cells. (A, B) Mean of tumor volume (A) and body weight of mice (B) measured at the indicated number of days after MDA-MB- 231HM and MDA-MB-468 cells were implanted into the mammary fat pad of mice. (C) Representative pictures of lung metastasis in PBS- (a), PA- (b), and PA-MSHA- (c) treated MDA-MB-231HM cell-transplanted mice. The number of mice with lung metastasis in all groups (d), the median number of metastatic nodules per mouse in MDA-MB-231HM cell line xenografts and the median size of the lung metastatic nodules of mice in all PBS-, PA-, and PA-MSHA-treated groups (e) were calculated. The black arrows indicate lung metastasis (a–c). (D) The expression levels of EGFR signaling proteins in PBS-, PA-, or PA-MSHA-treated xenografts. (E) The amount of VEGF (a) and MMP-9 (b) in the serum of PBS-, PA-, or PA-MSHA-treated mice was detected by ELISA. *Po0.05. Bars, s.d.

Article Snippet: In addition, the amounts of mouse VEGF and MMP-9 in PA-MSHA-treated PA-treated MDA-MB-231HM and MDAMB-468 5-week xenografts were also determined by an ELISA kit (Mouse VEGF SXR068, Mouse MMP9 SXR049 immunoassay kit, R&D Systems Inc.) in duplicate according to the manufacturer’s instructions.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay

Journal: Heliyon

Article Title: Aloe-emodin alleviates inflammatory bowel disease in mice by modulating intestinal microbiome homeostasis via the IL-4/IL-13 axis

doi: 10.1016/j.heliyon.2024.e34932

Figure Lengend Snippet: AE alleviates mice colitis induced by DSS. A : Changes in body weights of mice were monitored following treatment with different doses of AE in a mouse model of colitis induced by DSS. B : Intestinal inflammation marker LCN2 in feces. Levels of LCN2 in fecal samples collected from mice were measured as a marker of intestinal inflammation. C : H&E staining of colon tissue sections. Histological evaluation of colon tissue slices stained with H&E to assess tissue damage and inflammation. Representative images are shown. Data are shown as mean ± SD, ***P < 0.001: compared with control group; ##P < 0.01 and ###P < 0.001: compared with DSS group. Representative results from one out of three independent experiments.

Article Snippet: Additionally, fecal samples collected on day 15 were used for the detection of lipocalin-2 (LCN2) using an ELISA kit (RK-KOA0515; Rockland, Limerick, PA, USA).

Techniques: Marker, Staining, Control

Journal: Heliyon

Article Title: Aloe-emodin alleviates inflammatory bowel disease in mice by modulating intestinal microbiome homeostasis via the IL-4/IL-13 axis

doi: 10.1016/j.heliyon.2024.e34932

Figure Lengend Snippet: The content of LCN2 in control, DSS induced, and AE treated mice (ng/gfecal).

Article Snippet: Additionally, fecal samples collected on day 15 were used for the detection of lipocalin-2 (LCN2) using an ELISA kit (RK-KOA0515; Rockland, Limerick, PA, USA).

Techniques: Control